Essential Fish Habitat project status report

: Groundfish that associate with rugged seafloor types are difficult to assess with bottom-trawl sampling gear. Simrad ME70 multibeam echosounder (MBES) data and video imagery were collected to characterize trawlable and untrawlable areas, and to ultimately improve efforts to determine habitat-specific groundfish biomass. The data were collected during two acoustic-trawl surveys of the Gulf of Alaska (GOA) during 2011 and 2012 by NOAA Alaska Fisheries Science Center (AFSC) researchers. MBES data were collected continuously along the trackline, which included parallel transects (1-20 nmi spacing) and fine-scale survey locations in 2011. Video data were collected at camera stations using a drop camera system. Multibeamderived seafloor metrics were overlaid with the locations of previously conducted AFSC bottomtrawl (BT) survey hauls and 2011 camera stations. Generalized linear models were used to identify the best combination of multibeam metrics to discriminate between trawlable and untrawlable seafloor for the region of overlap between the camera stations or haul paths and the MBES data. The most discriminatory models were chosen based on the Akaike information criterion (AIC). The two best models were developed using data collected at camera stations with either oblique incidence backscatter strength (Sb) or mosaic Sb in combination with bathymetric position index and seafloor ruggedness and described over 54% of the variation between trawlable and untrawlable seafloor types. A map of predicted seafloor trawlability produced from the model using mosaic Sb and benthic-terrain metrics demonstrated that 58% of the area mapped (5,987 km2 ) had \u3e 50% probability of being trawlable and 42% of being untrawlable. The model predicted 69% of trawlable and untrawlable haul locations correctly. Successful hauls occurred in areas with 62% probability of being trawlable and haul locations with gear damage occurred in areas with a 38% probability of being trawlable. This model and map produced from multibeamderived seafloor metrics may be used to refine seafloor interpretation for the AFSC BT surveys and to advance efforts to develop habitat-specific biomass estimates for GOA groundfish populations

Cocktails and Brainwaves: Experiments with Complex and Subliminal Auditory Stimuli

The paper deals with the problem of processing acoustic signals originating from multiple sources in a potentially noisy environment. Previous research in speech processing and cognitive modelling has tended to concentrate on single sources and relatively noise free signals. Separating out different signals from a multitude of sources is a significant part of human auditory processing. In speech processing research, the problem we are dealing with is known as the cocktail party syndrome. The processing of polyphonic music involves similar challenges, and auditory scene analysis (ASA) has been proposed as a means of separating out component signals and identifying their sources. In subliminal auditory processing, a speech signal which is masked from conscious awareness by a music signal provides an extreme form of the multiple source problem and permits exploration of the boundary between conscious and unconscious auditory processing. The research presented employs machine learning and associative models to characterize and track individual signals, and uses electroencephalographic (EEG) analysis to more precisely characterize human processing of multimodal signals

Retrospective Study of Midazolam Protocol for Prehospital Behavioral Emergencies

Introduction: Agitated patients in the prehospital setting pose challenges for both patient care and emergency medical services (EMS) provider safety. Midazolam is frequently used to control agitation in the emergency department setting; however, limited data exist in the prehospital setting. We describe our experience treating patients with midazolam for behavioral emergencies in a large urban EMS system. We hypothesized that using midazolam for acute agitation leads to improved clinical conditions without causing significant clinical deterioration.Methods: We performed a retrospective review of EMS patient care reports following implementation of a behavioral emergencies protocol in a large urban EMS system from February 2014–June 2016. For acute agitation, paramedics administered midazolam 1 milligram (mg) intravenous (IV), 5 mg intramuscular (IM), or 5 mg intranasal (IN). Results were analyzed using descriptive statistics, Levene’s test for assessing variance among study groups, and t-test to evaluate effectiveness based on route.Results: In total, midazolam was administered 294 times to 257 patients. Median age was 30 (interquartile range 24–42) years, and 66.5% were male. Doses administered were 1 mg (7.1%) and 5 mg (92.9%). Routes were IM (52.0%), IN (40.8%), and IV (7.1%). A second dose was administered to 37 patients. In the majority of administrations, midazolam improved the patient’s condition (73.5%) with infrequent adverse events (3.4%). There was no significant difference between the effectiveness of IM and IN midazolam (71.0% vs 75.4%; p = 0.24).Conclusion: A midazolam protocol for prehospital agitation was associated with reduced agitation and a low rate of adverse events

AMP peptide targets tight junctions to protect and heal barrier structure and function in models of IBD.

Background: A peptide derived from Antrum Mucosal Protein (AMP)-18 (gastrokine-1) reduces the extent of mucosal erosions and clinical severity in mice with dextran sulfate sodium (DSS)-induced colonic injury. The present study set out to determine if AMP peptide was also therapeutic for immune- and cytokine-mediated mouse models of intestinal injury and inflammatory bowel diseases (IBD) by enhancing and stabilizing tight junctions (TJs). Methods: Therapeutic effects of AMP peptide were examined in interleukin-10 deficient and a T cell adoptive transfer models of colitis in immunodeficient recombinase activating gene-1 knock-out (RAG-1−/−) mice. Mechanisms by which AMP peptide enhances barrier function and structure were studied ex vivo using intestine and colon from mice given lipopolysaccharide (LPS), and in AMP-18 deficient mice given DSS. Results: In interleukin-10 deficient mice given piroxicam, AMP peptide enhanced recovery after weight loss, protected against colon shortening and segmental dilation, and reduced the colitis activity score. In the T cell transfer model, treatment with the peptide protected against colon shortening. In mice given LPS in vivo to induce gut injury, AMP peptide prevented the onset of, and reversed established intestinal hyperpermeability by targeting TJ proteins and perijunctional actin

The contribution of Chinese exports to climate change

Within 5 years, China's CO2 emissions have nearly doubled, and China may already be the world's largest emitter of CO2. Evidence suggests that exports could be a main cause for the rise in Chinese CO2 emissions; however, no systematic study has analyzed this issue, especially over time. We find that in 2005, around one-third of Chinese emissions (1700 Mt CO2) were due to production of exports, and this proportion has risen from 12% (230 Mt) in 1987 and only 21% (760 Mt) as recently as 2002. It is likely that consumption in the developed world is driving this trend. A majority of these emissions have largely escaped the scrutiny of arguments over “carbon leakage” due to the current, narrow definition of leakage. Climate policies which would make the developed world responsible for China's export emissions have both benefits and costs, and must be carefully designed to achieve political consensus and equity. Whoever is responsible for these emissions, China's rapidly expanding infrastructure and inefficient coal-powered electricity system need urgent attention

Thermalkalibacillus uzonensis gen. nov. sp. nov, a novel aerobic alkali-tolerant thermophilic bacterium isolated from a hot spring in Uzon Caldera, Kamchatka

A novel thermophilic, alkali-tolerant, and CO-tolerant strain JW/WZ-YB58T was isolated from green mat samples obtained from the Zarvarzin II hot spring in the Uzon Caldera, Kamchatka (Far East Russia). Cells were Gram-type and Gram stain-positive, strictly aerobic, 0.7-0.8 μm in width and 5.5-12 μm in length and produced terminal spherical spores of 1.2-1.6 μm in diameter with the mother cell swelling around 2 μm in diameter (drumstick-type morphology). Cells grew optimally at pH25°C 8.2-8.4 and temperature 50-52°C and tolerated maximally 6% (w/v) NaCl. They were strict heterotrophs and could not use either CO or CO2 (both with or without H2) as sole carbon source, but tolerated up to 90% (v/v) CO in the headspace. The isolate grew on various complex substrates such as yeast extract, on carbohydrates, and organic acids, which included starch, d-galactose, d-mannose, glutamate, fumarate and acetate. Catalase reaction was negative. The membrane polar lipids were dominated by branched saturated fatty acids, which included iso-15:0 (24.5%), anteiso-15:0 (18.3%), iso-16:0 (9.9%), iso-17:0 (17.5%) and anteiso-17:0 (9.7%) as major constituents. The DNA G+C content of the strain is 45 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW/WZ-YB58T is distantly (\u3c93% similarity) related to members of Bacillaceae. On the basis of 16S rRNA gene sequence, physiological and phenotypic characteristics, the isolate JW/WZ-YB58T (ATCC BAA-1258; DSM 17740) is proposed to be the type strain for the type species of the new taxa within the family Bacillaceae, Thermalkalibacillus uzoniensis gen. nov. sp. nov. The Genbank accession number for the 16S rRNA gene sequence is DQ221694. © Springer-Verlag 2006

Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy

Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.Fil: Wittstein, Kathrin. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Cordsmeier, Arne. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. Universitat Erlangen Nuremberg; AlemaniaFil: Lambert, Christopher. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Wendt, L.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Sir, Esteban Benjamin. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Weber, J.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Wurzler, N.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Petrini, Liliane E.. Via al Perato 15c; SuizaFil: Stadler, Marc. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemani